OA-7

Effects of phosphate on mineralization related gene expression of dental follicle cell

Sunao Sato¹, Masae Kitagawa², Shinji Iizuka¹, Kiyako Sakamoto¹, Hiroko Oka¹, Mutsumi Miyauchi¹,

Brian Foster³, Martha Somerman³, and Takashi Takata¹

¹Department of Oral Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University

²Center of Oral Clinical Examination, Hiroshima University Hospital

³Department of Periodontics, School of Dentistry, University of Washington

The mouse disordered in phosphate and pyrophosphate metabolism exhibits abnormalities of bone formation and marked increase in cementum formation, however precise mechanism is still unclear. To examine the role of phosphate in cementogenesis, we cultured follicle cells with phosphate and assayed expression of mineralization related genes. After 48H culture with 0-5mM phosphate, 3mM phosphate showed most increased BMP-2 and OPN expression. Then we did time course experiment (1-120H) with 3mM phosphate. Most increased BMP-2 and OPN expression was seen in 72H group. These results suggest that phosphate regulates the mineralization related gene expression of follicle cells.