OA-3

Geometry of artificial extracellular matrices: BMP-induced ectopic osteogenesis with three geometrically different titanium-webs

Youichi Uramoto¹, Hiroko Takita², Yoshinori Kuboki³ and Masato Tamura¹

¹Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University

²Central Research Institute, ³Koken Bioscience Institute

We examined whether geometry of titanium-web (TW) as the cell-substratum controls BMP-induced osteogenesis. Three geometrically different TW which were created by altering the diameter of the titanium fiber (8, 50 or 80 mm; TW-8, TW-50 or TW-80) were mixed with BMP-2 and implanted subcutaneously into the back of rats. The highest calcium and osteocalcin content of implants was found in TW-8 at 2 or 4 weeks after implantation. Histological observations indicated that the bone-like tissue volume in TW-8 was much higher than in TW-50 or TW-80. This study clearly demonstrated that a certain geometrical size (8 mm fibers) is most favorable for BMP-induced bone formation.