SNPs analysis of the enamerin
gene in severe dental caries of primary dentition period
Ken
Ouryouji, Yasuhiro Imamura, Yoshihisa Fujigaki, Hiroo Miyazawa and Pao-Li Wang
Department of Pediatric Dentistry*,
Department of Pharmacology**, Department of Hard Tissue Research,Graduate
An inherited factor is
one of the cause of dental caries. We examined the
relationship between SNPs of the enamerin
gene and severe dental caries of primary dentition period. The subjects were 120
unrelated individuals of which 75 were severe dental caries patients (dmft≧10) who
visited Matsumoto Dental University hospital, and 45 were healthy volunteers
serving as controls. The lingual cells from subjects were harvested with tooth
brush and DNAs were extracted. SNPs
analysis of the enamerin gene at the position+2452
was carried out a PCR-RFLP method using DNAs and the
specific primers. The results suggested that no significant difference was
observed between healthy and severe caries subjects. The other candidate genes
should be examined.