Effects of phosphate on mineralization related gene expression of dental follicle cell


Sunao Sato1, Masae Kitagawa2, Shinji Iizuka1, Kiyako Sakamoto1, Hiroko Oka1, Mutsumi Miyauchi1,

Brian Foster3, Martha Somerman3, and Takashi Takata1


1Department of Oral Maxillofacial Pathobiology, Graduate School of Biomedical Sciences, Hiroshima University

2Center of Oral Clinical Examination, Hiroshima University Hospital

3Department of Periodontics, School of Dentistry, University of Washington


The mouse disordered in phosphate and pyrophosphate metabolism exhibits abnormalities of bone formation and marked increase in cementum formation, however precise mechanism is still unclear. To examine the role of phosphate in cementogenesis, we cultured follicle cells with phosphate and assayed expression of mineralization related genes. After 48H culture with 0-5mM phosphate, 3mM phosphate showed most increased BMP-2 and OPN expression. Then we did time course experiment (1-120H) with 3mM phosphate. Most increased BMP-2 and OPN expression was seen in 72H group. These results suggest that phosphate regulates the mineralization related gene expression of follicle cells.