OA-5

RANKL induces the expression of carbonic anhydrase II, cathepsin K, and matrix metalloproteinase-9 in RAW264.7 cells

Kyosuke Fujisaki¹, Natsuko Tanabe^2,3, Naoto Suzuki^3,4, and Masao Maeno^2,3

¹ Department of Endodontics, Nihon University School of Dentistry

² Department of Oral Health Sciences, Nihon University School of Dentistry

³ Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry

⁴ Department of Biochemistry, Nihon University School of Dentistry

We examined the effects of RANKL and/or M-CSF in the presence of IL-1α or IL-1α alone, on the expression of carbonic anhydrase II (CAII), cathepsin K, matrix metalloproteinases-9 (MMP-9), M-CSF receptor (cfms), RANK, and c-Fos using RAW264.7 cells as osteoclast precursor. In the presence of IL-1α, CAII, cathepsin K, and MMP-9 expressions increased markedly in cells cultured with RANKL, or M-CSF plus RANKL, whereas their expressions were difficult to detect in cell culture with IL-1α alone and M-CSF. RANK and c-Fos expressions also increased in cells cultured with RANKL, or M-CSF plus RANKL in the presence of IL-1α, whereas c-fms expression was not change. These results indicated that the expression of CAII, cathepsin K, and MMP-9 in RAW264.7 cells is induced by not IL-1α and M-CSF but RANKL.