Geometry of artificial extracellular matrices: BMP-induced ectopic osteogenesis with three geometrically different titanium-webs


Youichi Uramoto1, Hiroko Takita2, Yoshinori Kuboki3 and Masato Tamura1


1Department of Biochemistry and Molecular Biology, Graduate School of Dental Medicine, Hokkaido University

2Central Research Institute, 3Koken Bioscience Institute


We examined whether geometry of titanium-web (TW) as the cell-substratum controls BMP-induced osteogenesis.  Three geometrically different TW which were created by altering the diameter of the titanium fiber (8, 50 or 80 mm; TW-8, TW-50 or TW-80) were mixed with BMP-2 and implanted subcutaneously into the back of rats.  The highest calcium and osteocalcin content of implants was found in TW-8 at 2 or 4 weeks after implantation. Histological observations indicated that the bone-like tissue volume in TW-8 was much higher than in TW-50 or TW-80. This study clearly demonstrated that a certain geometrical size (8 mm fibers) is most favorable for BMP-induced bone formation.