Properties of osteoinduction and BMP-2 release in functionally graded HAp


Junichi Tazaki1, Masaya Yamamoto2, Yasuhiko Tabata2, Toshiyuki Akazawa3,

Jun Hino1, Masaru Murata1, and Makoto Arisue1


1Oral and Maxillofacial Surgery, School of Dentistry, Health Sciences University of Hokkaido

2Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University

3Department of Materials Technology, Hokkaido Industrial Research Institute


The aim of this study is to investigate in vivo local BMP-2 PK and bone induction in two bioceramics blocks(3~3~3mm), based on different surface structures. Spongy and cortical bones in bovine femur were calcined at 800 by the step-wise calcinations to obtain bulk HAp (b-HAp). The functionally graded HAp (fg-HAp) was designed by the partial dissolution-precipitation method. We estimated the in vivo release profile of 125I–labeled BMP-2 and induction of hard tissues histologically. Specific nano-micro structures and biodegradation of fg-HAp is more effective for both BMP-2 retention and bone induction, compared to b-HAp, in the ectopic model.