Asian Science Seminar     ASS-4


Biological analysis of bone marrow stromal cells in vivo cell culture method


Mathieu Bernard Lefevre1, 2, Andrea Paola Rodriguez2, 3, Hidetsugu Tsujigiwa2, Hitoshi Nagatsuka2 and Noriyuki Nagai2

1Paris V University, France

2Department of Oral Pathology and Medicine. Graduate School of Medicine, Dentistry and Pharmaceutical Sciences. Okayama University. Okayama, Japan

3Department of Oral Pathology. Tucuman National University, School of Dentistry. Tucuman, Argentina


KUSA/A1 cells are immature mesenchymal stem cells isolated from bone marrow. These cells can differentiate to at least three types of mature cells like osteoblast, adipocyte and myoblast after treatment with 5-Azacytidine. Study showed that subcutaneous implantation of mature KUSA/A1 osteoblasts in diffusion chambers resulted in bone formation. It is well know that immature mesenchymal stem cells have better proliferation capabilities than mature cells. So, we hypothesized that the use of immature stem cells rather than mature cells could yield better results in tissue engineering due to the possibility of obtaining more cells to promote tissue repair.

For this purpose, we have evaluated an appropriate medium to maintain KUSA/A1 cells (kindly provided by Dr. Umezawa, Keio University) in their immature stage. In order to determine the ability of these immature KUSA/A1 cells to differentiate under the influence of the host microenvironment, immature KUSA/A1 cells in diffusion chamber have been implanted in intraperitoneal site in SCID mice. The results indicated that immature KUSA/A1 cells in vivo cell culture differentiated to osteoblasts and produced bone-like tissue.

This study supports that immature KUSA/A1 cells show osteogenic potential activity in in vivo culture method.